ABSTRACT
Objective To investigate the expression of SP 100 protein in ATRA-treated NB4 cells and its effect on pro-liferation in NB4 cells.Methods Q-PCR was employed to measure the expression of SP 100 mRNA;Western blot was used to detect the expression of SP 100 protein; Immunofluorescence was adopted to determine the location of SP100;Cell viability was analyzed by CCK 8;Flow cytometry was used for cell cycle analysis .Results ATRA may induce the expression of mRNA and protein of SP 100.ATRA changes the location of SP100 from a micro-punctate pattern into a punctate nuclear pattern in NB 4 cells.SP100-shRNA promotes the proliferation of NB 4 cells and in-creased the cells in G2/M phase.Conclusions The expression of SP100 was significantly increased in ATRA-treated NB4 cells, and SP100 may be involved in the regulation of proliferation activity of NB 4 cells.
ABSTRACT
Objective To explore the effect of comprehensive intervention on social function and quality of life of patients with bipolar disorder (BD) in maintenance treatment period.Methods A total of 83 patients with BD were assigned into intervention group (n=41) and control group (n=42).Based on the original drug treatment,the intervention group was given comprehensive intervention,including BD health education,drug self-management,serf-monitoring of symptoms,interpersonal skills,coping and relaxation skills,learning to seek help from medical staff and others.The control group was treated with simple drug therapy.Before grouping,at the end of the third month and twelfth month,Young Mania Rating Scale (YMRS),Hamilton Depression Scale 17 items (HAMD17),Social Disability Screening Schedule (SDSS),Short Form 36 items Health survey Questionnaire (SF-36) were assessed.Results There were no differences in YMRS,HAMD,SDSS total score between the two groups before grouping(P＞0.05).The score of YMRS in the intervention group ((0.59±1.45) was lower than that in the control group(2.07±3.87) at the end of the 12th month,the difference was statistically significant (P=0.024).The score of SDSS in the intervention group (1.63± 1.77,0.78± 1.78) were statistically lower than that in control group (3.57± 1.78,2.74± 1.27) at the end of the 3th and 12th month(P＜0.01).There were statistically differences between the two groups in rolephysical(RP) (t=2.858,P =0.005),role-emotional (RE) (t =2.956,P =0.005),social function (SF) (t =4.163,P＜0.01),vitality (VT) (t =5.150,P＜ 0.01),mental health (MH) (t =2.830,P=0.007),general health (GH) (t=4.055,P＜0.01),difference in reported health transition (HT) (t=-2.092,P=0.042)at the end of 3th month.The differences were statistically significant in RE (t =3.290,P =0.001),SF (t =2.876,P =0.006),VT(t=5.831,P＜0.01),MH(t=4.839,P＜0.01),GH(t=3.752,P＜0.01) at the end of 12th month between the two groups.Conclusion On the base of medicine treatment,comprehensive intervention can effectively improve the social function and life quality of patients with BD in maintenance treatment period.
ABSTRACT
Objective To explore the effect of comprehensive intervention on social function and quality of life of patients with bipolar disorder (BD) in maintenance treatment period.Methods A total of 83 patients with BD were assigned into intervention group (n=41) and control group (n=42).Based on the original drug treatment,the intervention group was given comprehensive intervention,including BD health education,drug self-management,serf-monitoring of symptoms,interpersonal skills,coping and relaxation skills,learning to seek help from medical staff and others.The control group was treated with simple drug therapy.Before grouping,at the end of the third month and twelfth month,Young Mania Rating Scale (YMRS),Hamilton Depression Scale 17 items (HAMD17),Social Disability Screening Schedule (SDSS),Short Form 36 items Health survey Questionnaire (SF-36) were assessed.Results There were no differences in YMRS,HAMD,SDSS total score between the two groups before grouping(P＞0.05).The score of YMRS in the intervention group ((0.59±1.45) was lower than that in the control group(2.07±3.87) at the end of the 12th month,the difference was statistically significant (P=0.024).The score of SDSS in the intervention group (1.63± 1.77,0.78± 1.78) were statistically lower than that in control group (3.57± 1.78,2.74± 1.27) at the end of the 3th and 12th month(P＜0.01).There were statistically differences between the two groups in rolephysical(RP) (t=2.858,P =0.005),role-emotional (RE) (t =2.956,P =0.005),social function (SF) (t =4.163,P＜0.01),vitality (VT) (t =5.150,P＜ 0.01),mental health (MH) (t =2.830,P=0.007),general health (GH) (t=4.055,P＜0.01),difference in reported health transition (HT) (t=-2.092,P=0.042)at the end of 3th month.The differences were statistically significant in RE (t =3.290,P =0.001),SF (t =2.876,P =0.006),VT(t=5.831,P＜0.01),MH(t=4.839,P＜0.01),GH(t=3.752,P＜0.01) at the end of 12th month between the two groups.Conclusion On the base of medicine treatment,comprehensive intervention can effectively improve the social function and life quality of patients with BD in maintenance treatment period.